Yeyiphi eyona Protocol ye-Centrifuging Blood yokuLungisa iPRF?

Kwiminyaka yakutshanje, iPlatelet-Rich Fibrin (PRF) iye yafumana ukuqondwa kwiimpawu zayo zokuvuselela kwaye isetyenziswa ngokubanzi kumazinyo, amathambo, kunye neenkqubo zokuthambisa. Kwiilabhoratri kunye neekliniki ezijonge ukulungiselela i-PRF, kubalulekile ukusebenzisa iisetingi ezifanelekileyo ze-centrifuge kunye nobuchule bokufumana iziphumo ezizezona zilungileyo. Eli nqaku linika isikhokelo sesinyathelo-nge-nyathelo ekulungiseleleni i-PRF kunye nokukhetha i-centrifuge engcono kakhulu kunye nezicwangciso ze-rotor.

Yintoni iPRF?

I-Platelet-Rich Fibrin (i-PRF) yiplatelet yesizukulwana sesibini esigxininise esityebileyo kwizinto zokukhula, i-cytokines, kunye ne-fibrin matrix ekhawuleza ukuphulukiswa kwezicubu kunye nokuvuselelwa. Ngokungafaniyo nePlatelet-Rich Plasma (PRP), i-PRF ayifuni naziphi na ii-anticoagulants okanye izongezo, iyenza ibe yi-100% yendalo ye-autologous biomaterial. Xa igazi li-centrifuged ukudala i-PRF, ifom ye-fibrin clot ibamba iiplatelet kunye ne-leukocytes, ngokuthe ngcembe ikhupha izinto zokukhula ukuze zivuselele ukuphilisa.

Amanyathelo okuLungisa iPRF

Ukulungiselela iPRF kufuna amanyathelo angundoqo ambalwa kunye nolawulo olululo lweeparamitha ze-centrifugation:

1.Ukuqokelelwa kweGazi

● Sebenzisa isirinji engenazintsholongwane ukutsala i-10-15 ml yegazi le-venous kumguli. Inkqubo yokuqokelela kufuneka yenziwe ngokukhawuleza kangangoko kunokwenzeka ukuphepha ukujika kwangaphambi kwexesha.

● Dlulisela igazi eglasini okanye kwiityhubhu zeplastiki ezingasebenziyo ngaphandle kwee-anticoagulants.

2.I-Centrifugation

● Ngoko nangoko beka imibhobho yegazi kwi-centrifuge ukuthintela ukumila kwamahlwili.

● Seta i-centrifuge ngokusekwe kwiprothokholi oyikhethileyo (jonga iinkcukacha ezingezantsi malunga ne-centrifugal force kunye nobude bexesha).

3.Ukukhupha iPRF

● Emva kwe-centrifugation, umaleko otyheli, onjengejeli uya kumila kumbindi wombhobho, owahlula iplasma kunye neeseli ezibomvu zegazi.

● Khupha ngobunono lo maleko we-PRF usebenzisa i-forceps okanye i-spatula, ukuthintela nakuphi na ukuxubana nomaleko weseli ebomvu engezantsi.

UKhetho lwe-PRF Centrifuge

Ukukhetha i-centrifuge eyiyo kubalulekile kulungiselelo olululo lwePRF. Nantsi eminye imiba ebalulekileyo:

● I-Speed ​​​​Speed ​​​​Range ye-Centrifuge: I-centrifuge kufuneka ikwazi ukufikelela kwisantya esisezantsi ukuya kwimodareyitha (malunga ne-2,000-3,000 RPM) kunye nezicwangciso zokuguquguquka kwamandla (RCF).

● Ukulawula Ubushushu: I-centrifuge epholileyo inokuba luncedo ukugcina ubushushu begazi kunye nokunciphisa i-clotting ngaphambi kokusekwa kwe-PRF.

● Iimodeli ze-PRF Centrifuge: Ii-centrifuges ze-PRF ezikhethekileyo ezifana ne-MKE TD5B kwaye BYL-5R I-PRF Centrifuge ilungile njengoko iyilelwe ukuphatha iiprothokholi ze-PRF ngokufanelekileyo ngesantya, ixesha, kunye nokukhethwa kwe-rotor.

I-Horizontal vs. Fixed-Angle Rotor Centrifuges

Ukhetho phakathi kweerotha ezithe tye kunye nezisisigxina zinefuthe kumgangatho wePRF:

● I-Horizontal Rotor: Imibhobho ibekwe ngokuthe tye, ivumela iinxalenye zegazi ukuba zihlale kumaleko ahlukeneyo. Iirotor ezithe tyaba zifanelekile kwi-PRF njengoko zibonelela ngokuhlukana okufanayo kwamacandelo egazi, ukwandisa ukubunjwa kwehlwili ye-fibrin.

● I-Angle-Angle Rotor: Iityhubhu zine-angle, ezinokuthi zikhokelela ekuhlukaneni komgangatho ongaphantsi. Ngelixa ii-centrifuges ezisisigxina zihlala zisetyenziselwa izicelo ezinesantya esiphezulu, azisebenzi kangako ekufezekiseni ulwahlulo oluchanekileyo olufunekayo kwi-PRF.

Ngokubanzi, i-rotor ethe tyaba iyacetyiswa ukulungiselela i-PRF ngenxa yobuchule bayo bokwenza iileya ezichazwe kakuhle, ukuqinisekisa ukuxinwa okuphezulu kwefibrin kwimveliso yokugqibela.

isiphelo

Ukusebenzisa ezona protocol ze-centrifuge kunye nezixhobo kubalulekile kulungiselelo oluyimpumelelo lwePRF. I-rotor centrifuge ethe tyaba enesethingi enokwenziwa ngokwezifiso inika ezona ziphumo zisebenzayo kunye nezihambelanayo. Ngokukhetha okufanelekileyo PRF centrifuge, ngokulandela i-centrifugal force ekhuthazwayo kunye nezicwangciso zexesha, kunye nokusebenzisa iindlela ezichanekileyo zokulungiselela, iilebhu kunye neekliniki zinokuvelisa i-PRF ephezulu yokuxhasa izicelo ezahlukeneyo zonyango.

Ngolwazi oluthe kratya malunga nokukhetha i-PRF centrifuge efanelekileyo, zive ukhululekile ukufikelela, okanye ujonge iimodeli zethu ze-centrifuge ezilungiselelwe ngokukodwa izicelo zePRF.